Publications of Alkaloid biosynthesis and bioproduction Group

2025

• Geissoschizine Cyclase: A Discovery Revolutionizing the Understanding and Synthesis of Pharmacologically Important Monoterpenoid Indole Alkaloids

2024

• A GAME changer in steroidal metabolite biosynthesis
• Genome-based discovery of pachysiphine synthases in Tabernaemontana elegans

2023

• The Rauvolfia tetraphylla genome suggests multiple distinct biosynthetic routes for yohimbane monoterpene indole alkaloids in monoterpene indole alkaloid synthesis
• Biosynthesis of natural and halogenated plant monoterpene indole alkaloids in yeast
• Medicinal plants enter the single-cell multi-omics era
• Gaining access to acetyl-CoA by peroxisomal surface display.
• The evolutionary pattern of cocaine and hyoscyamine biosynthesis provides strategies to produce tropane alkaloids
• Spatial localization of monoterpenoid indole alkaloids in Rauvolfia tetraphylla by high resolution mass spectrometry imaging
• Convergent evolution for antibiotic biosynthesis in bacteria and animals

2022

• An updated version of the Madagascar periwinkle genome
• Single-cell RNA sequencing provides a high-resolution roadmap for understanding the multicellular compartmentation of specialized metabolism
• Emerging mechanistic insights into the regulation of specialized metabolism in plants
• The high osmolarity glycerol (HOG) pathway in fungi
• Boosting lignan-precursor synthesis in yeast cell factories through co-factor supply optimization
• Genome Assembly of the Medicinal Plant Voacanga thouarsii
• The Vinca minor genome highlights conserved evolutionary traits in monoterpene indole alkaloid synthesis
• Computer-Assisted Design of Sustainable Syntheses of Pharmaceuticals and Agrochemicals from Industrial Wastes
• A microbial supply chain for production of the anti-cancer drug vinblastine published in NATURE!
• Identification of a second 16-hydroxytabersonine-O-methyltransferase suggests an evolutionary relationship between alkaloid and flavonoid metabolisms in Catharanthus roseus
• A new path for terpenoid biosynthesis
• Towards a Better Understanding of Toxin Biosynthesis in Seaweeds
• More than a Catharanthus plant: A multicellular and pluri-organelle alkaloid-producing factory
• Social amoebae as a new chassis for drug production
• Chromosome-scale genomes throw light on plant drug biosynthesis
• RNA-seq Analysis of Monoterpene Indole Alkaloid Biosynthetic Pathway Elucidation in Catharanthus roseus
• Predicting Monoterpene Indole Alkaloid-Related Genes from Expression Data with Artificial Neural Networks
• An Optimized Electroporation Procedure for Genetic Transformation of Candida auris
• A Rapid and Efficient Vacuum-Based Agroinfiltration Protocol for Transient Gene Overexpression in Leaves of Catharanthus roseus
• RNA In Situ Hybridization of Paraffin Sections to Characterize the Multicellular Compartmentation of Plant Secondary Metabolisms
• ARResting cytokinin signaling for salt-stress tolerance
• Marine drugs: Biology, pipelines, current and future prospects for production

2021

•  Kulagina N, Besseau S, Godon C, Goldman GH, Papon N,  Courdavault V. ^.Yeasts as Biopharmaceutical Production Platform. Front. Fungal Biol. 2021 : https://doi.org/10.3389/ffunb.2021.733492
• Koudounas K, Guirimand G, Rojas Hoyos LF, et al. Tonoplast and Peroxisome Targeting of γ-tocopherol N-methyltransferase Homologs Involved in the Synthesis of Monoterpene Indole Alkaloids [published online ahead of print, 2021 Nov 10]. Plant Cell Physiol. 2021;pcab160. doi:10.1093/pcp/pcab160
• Kulagina N, Perrin J, Besseau S, Courdavault V. Efficient Terpene Production by Marine Thraustochytrids: Shedding Light on the Thermodynamic Driving Force. mBio. 2021;12(5):e0197621. doi:10.1128/mBio.01976-21
• Yamamoto K, Grzech D, Koudounas K, et al. Improved virus-induced gene silencing allows discovery of a serpentine synthase gene in Catharanthus roseus. Plant Physiol. 2021;187(2):846-857. doi:10.1093/plphys/kiab285
• Méteignier LV, Papon N, Courdavault V. Plant to Insect Horizontal Gene Transfer: Empowering Whiteflies. Trends Genet. 2021;37(8):688-690. doi:10.1016/j.tig.2021.04.007
• Kulagina N, Guirimand G, Melin C, et al. Enhanced bioproduction of anticancer precursor vindoline by yeast cell factories [published online ahead of print, 2021 Jul 24]. Microb Biotechnol. 2021;10.1111/1751-7915.13898. doi:10.1111/1751-7915.13898
• Stander EA, Dugé de Bernonville T, Papon N, Courdavault V. Computational biotechnology guides elucidation of the biosynthesis of the plant anticancer drug camptothecin. Comput Struct Biotechnol J. 2021;19:3659-3663. Published 2021 Jun 22. doi:10.1016/j.csbj.2021.06.028
• Lemos Cruz P, Kulagina N, Guirimand G, et al. Optimization of Tabersonine Methoxylation to Increase Vindoline Precursor Synthesis in Yeast Cell Factories. Molecules. 2021;26(12):3596. Published 2021 Jun 11. doi:10.3390/molecules26123596
• Courdavault V, O'Connor SE, Jensen MK, Papon N. Metabolic engineering for plant natural products biosynthesis: new procedures, concrete achievements and remaining limits [published online ahead of print, 2021 May 10]. Nat Prod Rep. 2021;10.1039/d0np00092b. doi:10.1039/d0np00092b
• Kulagina N, Besseau S, Papon N, Courdavault V. Peroxisomes: A New Hub for Metabolic Engineering in Yeast. Front Bioeng Biotechnol. 2021;9:659431. Published 2021 Apr 7. doi:10.3389/fbioe.2021.659431
• Carqueijeiro I, Koudounas K, Dugé de Bernonville T, et al. Alternative splicing creates a pseudo-strictosidine β-d-glucosidase modulating alkaloid synthesis in Catharanthus roseus. Plant Physiol. 2021;185(3):836-856. doi:10.1093/plphys/kiaa075
• Perrin J, Kulagina N, Unlubayir M, et al. Exploiting Spermidine N-Hydroxycinnamoyltransferase Diversity and Substrate Promiscuity to Produce Various Trihydroxycinnamoyl Spermidines and Analogues in Engineered Yeast. ACS Synth Biol. 2021;10(2):286-296. doi:10.1021/acssynbio.0c00391
• Stander EA, Papon N, Courdavault V. Puzzling Out the Colchicine Biosynthetic Pathway. ChemMedChem. 2021;16(4):621-623. doi:10.1002/cmdc.202000633
• Kulagina N, Papon N, Courdavault V. Microbial Cell Factories for Tetrahydroisoquinoline Alkaloid Production. Chembiochem. 2021;22(4):639-641. doi:10.1002/cbic.202000579
• Guirimand G, Kulagina N, Papon N, Hasunuma T, Courdavault V. Innovative Tools and Strategies for Optimizing Yeast Cell Factories. Trends Biotechnol. 2021;39(5):488-504. doi:10.1016/j.tibtech.2020.08.010
• Courdavault V, Cassereau J, Papon N. Engineered Microbes for Producing Anticholinergics. Chembiochem. 2021;22(8):1368-1370. doi:10.1002/cbic.202000757