An Optimized Electroporation Procedure for Genetic Transformation of Candida auris
Santé-Sciences-Technologie,
Pharmacie,
Date(s)
du 23 février 2022 au 23 février 2025
Lieu(x)
Site Grandmont
EA2106 BBV - Biomolécules et Biotechnologies végétales
In this Chapter of the "Catharanthus roseus, Methods and Protocols" book, we provide a reliable transformation protocol based on an efficient electroporation procedure and the use of a mycophenolic acid resistance marker.
An Optimized Electroporation Procedure for Genetic Transformation of Candida auris
from :Kasir D, Osman M, Hamze M, Bouchara JP, Courdavault V, Papon N. Methods Mol Biol. 2022;2517:89-94. doi: 10.1007/978-1-0716-2417-3_7.
Abstract
Candida auris is responsible for recent outbreaks with significant mortality in hospitalized and long-term care patients. As a highly transmissible and multidrug-resistant fungal pathogen, genetic tools are urgently needed for deciphering mechanisms involved in the host-pathogen interactions and potentially identifying new fungal targets for therapeutic development. Here, we provide a reliable transformation protocol based on an efficient electroporation procedure and the use of a mycophenolic acid resistance marker.