Partager
Actualité

An Optimized Electroporation Procedure for Genetic Transformation of Candida auris

  • Santé-Sciences-Technologie,
  • Pharmacie,
Date(s)

du 23 février 2022 au 23 février 2025

Lieu(x)

Site Grandmont

EA2106 BBV - Biomolécules et Biotechnologies végétales

In this Chapter of the "Catharanthus roseus, Methods and Protocols" book, we provide a reliable transformation protocol based on an efficient electroporation procedure and the use of a mycophenolic acid resistance marker.

An Optimized Electroporation Procedure for Genetic Transformation of Candida auris


from :Kasir D, Osman M, Hamze M, Bouchara JP, Courdavault V, Papon N. Methods Mol Biol. 2022;2517:89-94. doi: 10.1007/978-1-0716-2417-3_7.

Abstract

Candida auris is responsible for recent outbreaks with significant mortality in hospitalized and long-term care patients. As a highly transmissible and multidrug-resistant fungal pathogen, genetic tools are urgently needed for deciphering mechanisms involved in the host-pathogen interactions and potentially identifying new fungal targets for therapeutic development. Here, we provide a reliable transformation protocol based on an efficient electroporation procedure and the use of a mycophenolic acid resistance marker.

Keywords: Candida auris; Drug resistance marker; Fluorescent protein; Mycophenolic acid; Transgenesis.