Trans-Resveratrol, a natural polyphenol found in various plant species, has gained significant attention due to its potential health-promoting properties. This article reviews the biological activities attributed to trans-Resveratrol, its dietary sources, and the analytical methods employed for its quantification. The compound antioxidant, anti-inflammatory, anticancer, cardioprotective, and neuroprotective activities are discussed in detail. Furthermore, the sources of trans-Resveratrol, including grapes, berries, and certain nuts, are examined in terms of their content variability and factors influencing production. Various chromatographic, spectroscopic, and immunoassay methods for the analysis of trans-Resveratrol in different matrices are also explored. This comprehensive overview underscores the significance of trans-Resveratrol as a potential molecule for enhancing human health and longevity.